Figure 4. IKKβ in myeloid cells regulates male and female responses to intraperitoneal sensitization to allergen.

(A) Cytokine responses in spleen tissues after oral or intraperitoneal sensitization. Spleens were collected 16 hours after intraperitoneal or oral sensitization and cytokine mRNA responses were determined by Real-time RT-PCR. (B) IKKβ^ΔMye mice were sensitized on days 0 and 7 by intraperitoneal injection of ovalbumin (100 μg) and cholera toxin (1 μg) or by oral administration of ovalbumin (1 mg) and cholera toxin (10 μg). Blood was collected on day 14. OVA-specific IgE, IgA isotypes and IgG subclass were measured by ELISA. The results are expressed as the log₂ titers ± one SD of two experiments with five mice per group.