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. 2014 Mar 25;9(3):e92647. doi: 10.1371/journal.pone.0092647

Figure 1. The mRNA and protein expression of phosphatidylethanolamine N-methyltransferase (Pemt).

Figure 1

a. The results of the quantitative RT-PCR assay for Pemt mRNA in various tissues of C57BL/6JJcl mice (n = 4). b. The Western blot analysis of Pemt in the kidney and liver tissues of Pemt+/+ mice and Pemt−/− C57BL/6JJcl mice. c–f. Immunoperoxidase staining for Pemt in the kidney tissue of Pemt+/+ mice and Pemt−/− mice. Immunoreactivity was seen in the proximal tubules and glomerular cells in streptozotocin (STZ)-treated Pemt+/+ mice (c), but not in control Pemt+/+ mice (e) or Pemt−/− mice (d and f). Bars = 50 μm (c–f).