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. 2014 Mar 25;9(3):e92647. doi: 10.1371/journal.pone.0092647

Figure 3. The results of the histochemical and morphometric analyses of streptozotocin (STZ)-treated diabetic Pemt+/+ and Pemt−/− C57BL/6JJcl mice.

Figure 3

The Pemt+/+ and Pemt−/− mice were treated with citrate buffer (CON) or streptozotocin (STZ). a–d. Periodic acid-Schiff stain, e–h. immunofluorescence staining for type IV collagen, i–l. immunofluorescence staining for TGF-β, m. the glomerular area (μm2), n. the mesangial matrix index (%) and o. the type IV collagen positive area/glomerular area (%). The glomerular hypertrophy, mesangial area and type IV collagen positive area were significantly reduced in Pemt−/− (STZ) mice compared with Pemt+/+ (STZ) mice. Bars = 50 μm (a–l). ##P<0.01, #P<0.05 v.s. Pemt+/+ (CON). **P<0.01, *P<0.05 v.s. Pemt+/+ (STZ).