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. 2014 Mar 25;9(3):e92647. doi: 10.1371/journal.pone.0092647

Figure 7. Apoptosis in the streptozotocin (STZ)-treated diabetic Pemt+/+ and Pemt−/− C57BL/6JJcl mice and in mProx24 cells treated with MISSION shRNA lentivirus transduction particles for Pemt (shRNA-Pemt) and Non-Target shRNA control lentivirus transduction particles (shRNA-CON).

Figure 7

a. and b. The results of the in situ TUNEL assay in Pemt−/− (STZ) and Pemt+/+ (STZ) mice. Bars = 100 μm. c. The quantification of apoptotic cells in the kidney cortex. The number of TUNEL-positive cells was significantly reduced in the Pemt−/− (STZ) mice compared with Pemt+/+ (STZ) mice. #P<0.05; STZ-treated group (STZ) v.s. citrate buffer treated control group (CON). *P<0.05; Pemt+/+ (STZ) v.s. Pemt−/− (STZ). d. The results of the Western blot analyses of caspases 3 and 7 in the renal cortex tissues of Pemt+/+ and Pemt−/− mice. e. The increase in cleaved caspases 3 and 7 was ameliorated by the deficiency of Pemt in STZ-treated mice. ##P<0.01, #P<0.05 v.s. Pemt+/+ (CON). **P<0.01, *P<0.05 v.s. Pemt+/+ (STZ).