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Cardiomyocytes from sham group and after preconditioning were subjected to hypoxia (5 hours) during the first and second window of protection in order to compare the survival between both groups and demonstrate the effect of preconditioning. Cell survival is given in percent of seeded cells. Whereas unpreconditioned cells were diminished to 48±2%, isoflurane preconditioned cells reached a survival ratio of 65±5% (n = 6 per group, p = 0.0061).
