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Cardiomyocytes were incubated with 100/ml recombinant mouseMIF for the indicated time points. Relative activation PKCε (A) AMPK (B), JNK1 (C) and JNK2 (D) was assessed via western blotting. Band intensities were normalized to actin. Data represented means ± SEM of at least 3 independent experiments. *(**) = p<0.05 (0.01) vs. baseline.
