Table 1. Summary of proteins differentially expressed in mouse muscle tissue infected by Chikungunya virus.
| Spot intensity Mean± SD | ||||||||||||
| S.No | Spot ID | Protein Identified | Accessiona | Mowse Scoreb PMF/MS-MS | % Covc | Peptides Matched (searched) | Mr/pI theoretical | Mr/pI observed | Mock Infected | CHIKV Infected | Fold change Mean± SD | P value |
| 1 | M1 | Vimentin | P20152 | 290/530 | 77 | 59 (132) | 53.68/5.06 | 63/4.9 | 5.8±1.7 | 18.5±6.4 | 3.15±0.41 | 8×10−3 |
| 2 | M2 | a.Albumin b.Kininogen | P07724 O08677 | 132/NA 86/77 | 59 51 | 36 (119) 31 (119) | 53.17/4.88 | 95/5.2 | 3.6±1.2 | 8.2±1.7 | 2.6±0.82 | 1×10−2 |
| 3 | M3 | Vimentin | P20152 | 241/241 | 85 | 61 (176) | 53.5/5.06 | 70/5.2 | 5.4±2.3 | 26±11.4 | 4.5±2.3 | 3×10−2 |
| 4 | M4 | Kininogen | O08677 | 105/NS | 56 | 28 (147) | 47.8/5.74 | 95/5.2 | 6.1±1.3 | 14.9±1.7 | 2.6±0.9 | 9×10−3 |
| 5 | M5 | Vimentin | P20152 | 214/73 | 73 | 54 (173) | 51.5/4.9 | 75/5.0 | 6.9±1.6 | 17.6±5.8 | 2.6±0.7 | 1×10−2 |
| 6 | M6 | Haptoglobin | Q3UBS3 | 64/117 | 54 | 21 (129) | 38.8/6.08 | 52/5.0 | 0 | 5.4±1.4 | DIV/0e | 6×10−3 |
| 7 | M7 | Myosin | Q5SX40 | 120/58 | 48 | 95 (167) | 60.9/5.4 | 92/5.4 | 4.9±1.9 | 12.2±3.0 | 2.3±0.8 | 1×10−2 |
| 8 | M8 | Vimentin | P20152 | 262/219 | 89 | 65 (162) | 51.5/4.96 | 72/5.3 | 27.7±4.3 | 52±9.2 | 2±0.6 | 5×10−3 |
| 9 | M9 | Vimentin | Q5FWJ3 | 265/474 | 89 | 77 (181) | 53.6/5.06 | 72/5.3 | 16.2±2.5 | 35.9±8.1 | 2.3±0.9 | 5×10−3 |
| 10 | M10 | Vimentin | P20152 | 217/337 | 83 | 64 (178) | 53.6/5.06 | 72/5.4 | 30±1.6 | 101±37.4 | 3.4±1.2 | 9×10−3 |
| 11 | M11 | Actin | P60710 | 160/215 | 82 | 37 (125) | 41.7/5.3 | 57/5.6 | 17.5±4.3 | 36.4±3.9 | 2.3±0.9 | 1×10−3 |
| 12 | M12 | a.Albumin b.Hemopexin | P07724 Q91X72 | 154/NA 98/417 | 71 56 | 54 (164) 27 (164) | 50.51/7.32 | 95/6.3 | 41.5±12 | 81±27.05 | 1.9±0.04 | 4×10−2 |
| 13 | M13 | a.Hemopexin b.Albumin | Q91X72 P07724 | 152/404 114/NA | 63 69 | 31 (126) 46 (126) | 51.3/7.92 | 95/6.4 | 17.5±6.8 | 53±14.6 | 3.4±1.8 | 4×10−3 |
| 14 | M14 | Hemopexin | Q91X72 | 170/55 | 51 | 25 (108) | 51.3/7.92 | 95/6.5 | 13±7.9 | 40±12.2 | 3.6±1.2 | 9×10−3 |
| 15 | M15 | Hemopexin | Q91X72 | 211/115 | 58 | 29 (111) | 51.3/7.92 | 95/6.7 | 5.67±0.7 | 35.5±6.3 | 6.4±1.9 | 2×10−2 |
| 16 | M16 | Glucose regulated protein | P27773 | 203/421 | 69 | 45 (170) | 56.59/5.78 | 72/6.4 | 21.4±3.6 | 40.1±6.8 | 1.9±0.4 | 2×10−3 |
| 17 | M17 | Pyruvate kinase isozyme M2 | P52480 | 120/84 | 83 | 35 (159) | 43.1/5.88 | 58/6.4 | 3.9±0.43 | 11.5±3.2 | 3.0±1.1 | 3×10−3 |
| 18 | M18 | Fibrinogen | Q3TGR2 | 110/124 | 66 | 42 (182) | 54.7/6.68 | 78/6.7 | 12±1.7 | 22±1.0 | 1.85±0.18 | 3×10−2 |
| 19 | M19 | Apolipoprotein A-1 | Q3V2G1 | 120/268 | 60 | 24 (147) | 30.66/5.64 | 34/5.9 | 18.7±7.1 | 66.3±4.0 | 3.9±1.2 | 2×10−5 |
| 20 | M20 | Peroxiredoxin-6 | O08709 | 176/306 | 88 | 30 (119) | 24.7/5.72 | 36/6.5 | 7.2±0.9 | 23.6±5.9 | 3.8±0.5 | 1×10−4 |
| 21 | M21 | Heat shock protein 25 | P14602 | 117/160 | 71 | 18 (110) | 23/6.12 | 36/6.7 | 11.4±3.8 | 40.6±10 | 3.8±1.6 | 9×10−3 |
| 22 | M22 | Rho GDP dissociation inhibitor 2 | Q61599 | 92/37 | 81 | 17 (97) | 22.7/4.97 | 34/5.1 | 3.4±0.7 | 10±0.96 | 3.0±0.6 | 3×10−5 |
| 23 | M23 | Actin | P60710 | NS/55 | NA | NA | 39/5.8 | 26/5.3 | 0 | 7.6±1.2 | DIV/0e | 1×10−5 |
| 24 | M24 | Actin related protein | Q6ZQK2 | 64/90 | 37 | 65 (180) | 191.2/6.15 | 26/6.3 | 0 | 4.7±0.95 | DIV/0e | 5×10−4 |
| 25 | M25 | NAd | NA | NA | NA | NA | NA | NA | 23.8±5.3 | 94.5±11 | 4.1±0.7 | 2×10−5 |
| 26 | M26 | Tropomyosin alpha chain | P58772 | 167/420 | 70 | 35 (129) | 32.8/4.6 | 50/4.8 | 512.6±22 | 208±15 | 0.4±0.3 | 5×10−3 |
| 27 | M27 | Tropomyosin alpha chain | P58772 | 126/323 | 69 | 31 (142) | 32.6/4.8 | 45/4.9 | 637±128 | 284.5±52 | 0.5±0.1 | 2×10−3 |
a = Swiss Prot accession number. b = By MASCOT. c = % Coverage of the identified sequence. NAd = not available. DIV/0e = Divided by zero. Sopt ID represents the number on 2-DE gels. NS = Non significant.