Skip to main content
. 2014 Mar 25;9(3):e92741. doi: 10.1371/journal.pone.0092741

Figure 2. AP-1 is involved in the potentiation of ICAM-1 expression by adiponectin.

Figure 2

OASFs were pretreated for 30(3 μM) or tanshinone IIA (5 μM) followed by stimulation with adiponectin (3 μg/ml) for 24 h, and ICAM-1 expression was examined by qPCR (A) and flow cytometry (B). OASFs were transfected with c-Jun siRNA or c-fos siRNA for 24 h followed by stimulation with adiponectin (3 μg/ml) for 24 h, and ICAM-1 expression was examined by qPCR (C) and flow cytometry (D). (E) OASFs were incubated with adiponectin (3 μg/ml) for the indicated time intervals and c-Jun phosphorylation was determined by western blot. Results are expressed as the mean ± S.E. *p<0.05, compared to basal expression levels. #p<0.05, compared to expression levels in the adiponectin-treated group.