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. 2014 Mar 25;9(3):e92741. doi: 10.1371/journal.pone.0092741

Figure 5. The LKB1, CaMKII, and AMPK signaling pathway is involved in adiponectin-induced AP-1 activation.

Figure 5

OASFs transiently transfected with AP-1-luciferase plasmid for 24 h and then pretreated with Ara A and compound C (A) for 30 min or cotransfected with LKB1, CaMKII, and AMPKα1 siRNA (B) for 24 h before incubation with adiponectin for 24 h. Luciferase activity was measured, and the results were normalized to the β-galactosidase activity. OASFs were transfected with LKB1, CaMKII, or AMPKα1 siRNA for 24 h followed by stimulation with adiponectin (3 μg/ml), and c-Jun phosphorylation and c-Jun binding to the ICAM-1 promoter were examined by western blot (C) and chromatin immunoprecipitation assay (D). Results are expressed as the mean ± S.E. *p<0.05, compared to basal expression levels. #p<0.05, compared to expression levels in the adiponectin-treated group.