Skip to main content
. 2014 Mar 25;9(3):e92857. doi: 10.1371/journal.pone.0092857

Figure 6. The effects of neutralizing antibodies to LYVE-1 on LMW-HA-induced PKC/MAPK activation.

Figure 6

(A) Phosphorylated versions and total of PKC and ERK were analyzed by Western blotting. SVEC4-10 cells were incubated with LMW-HA (b, 3.13 μg/ml) or serum free medium only (a) for 20min. In some cases, cells were pre-treated with neutralizing anti-LYVE-1 antibodies (d) or isotype IgG (c) for 12h and stimulated with LMW-HA (3.13 μg/ml) for another 20min. After stimulation, cells were then analyzed by Western blotting. Total PKCα and ERK1/2 in addition to GAPDH were used for normalization. Data are representative of three independent experiments. (B) Expression ratios were analyzed by Image Pro Plus 6.0 software. **, p<0.01. ***, p<0.001.