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. 2014 Mar 25;9(3):e92837. doi: 10.1371/journal.pone.0092837

Figure 3. Analytical threshold.

Figure 3

The purpose of this threshold is to distinguish allelic signal from background noise. Applying the threshold (red line) reduces the quantitative peaks to all-or-none putative allele events (blue bars). The analytical threshold operation eliminates individual peak heights, as well as their collective pattern.