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. 2014 Mar 25;9(3):e92973. doi: 10.1371/journal.pone.0092973

Figure 3. Establishment of secondary MEFs and secondary iPSCs.

Figure 3

(A) Morphology of secondary MEFs. Scale bar, 10 μm. (B and C) Numbers of secondary iPSC colonies. (D) Morphology of secondary iPSC colonies. Scale bar, 50 μm. (E) Morphology of secondary iPSC cell lines. Scale bar, 10 μm. (F and G) Comparison of endogenous expressions of the four reprogramming factors, transgenes, and ESC-specific genes between primary and secondary iPSCs.