Figure 6. BV modulates GSK3β-mediated suppression of Foxp3⁺ Treg cells in vitro.

(A), Representative flow cytometric results of Foxp³⁺ Treg cells in cultured splenocytes after incubation with BV or transfection with GSK3β siRNA for 72 h. (B), Both BV (100 μg/mL) and GSK3β siRNA (100 pmol/mL) were shown to significantly increase the frequencies of Foxp3+ Treg cells in cultured splenocytes. (C), Representative western blot results of GSK3β expression in cultured splenocytes after incubation with BV or transfection with GSK3β siRNA for 72 h. (D), Both BV and GSK3β siRNA were shown to significantly decrease the expression of GSK3β in cultured splenocytes (n = 3). Each sample was tested for 3 times and averaged, all values were expressed as mean ± SD. * P<0.05.