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. 2014 Mar 25;9(3):e92875. doi: 10.1371/journal.pone.0092875

Figure 4. Functional analysis of yellow through RNAi.

Figure 4

Female and male pupae were injected twice with 1 μl of PBS (A and E) or 10 μg of either GFP (B and F) or yellow (C, D and G) dsRNA in 1 μl of PBS. Pupae injected with PBS or GFP dsRNA exhibited normal body color (A, B, E and F). Injection with yellow dsRNA affected pigmentation in females (C and D) but not in males (G). The effect of yellow RNAi varied among individual females. One female exhibited strongly impaired black pigmentation throughout the body (C), some exhibited only partially or weakly reduced coloration (D), while others exhibited an almost normal body color. The effect of RNAi on body color was evaluated using a HSB color model (H). The hue angle of yellow-RNAi females was significantly decreased and their brightness was significantly increased (see Fig. S3).