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. 2014 Mar 25;9(3):e93144. doi: 10.1371/journal.pone.0093144

Figure 2. Sera from IBS patients show high levels of monocytes chemotactic protein-1 (MCP-1) and macrophage inflammatory protein-1β (MIP-1β).

Figure 2

Sera (250 μL) from IBS patients (44 idiopathic IBS, 16 post-infectious IBS, and 40 healthy volunteers) were analyzed for the presence of 36 biomarkers using the Proteome Profiler Human Cytokine Array Panel A kit (R&D Systems, Minneapolis, MN). Following chemiluminescent detection and processing, the intensities of the blots were determined using ImageJ (National Institutes of Health, Bethesda, MD) and expressed as pixel density. Logistic regression analysis indicated that the amounts of MCP-1 and MIP-1β in the sera were significantly higher in both ID-IBS and PI-IBS (p-values 0.003 and 0.010, respectively) when compared to that of the healthy volunteers. The odds ratios of association between IBS and overexpression of MCP-1 and MIP-1β were 1.054 (95% CI: 1.011–1.100), 1.021 (95% CI: 1.048–1.061), respectively.