Figure 4. (Top) The mRNA levels of NAD(P)H quinone oxidoreductase 1 (Nqo1) and glutamate-cysteine ligase, catalytic subunit (Gclc) in Nrf2-null, wild-type and Keap1-HKO mice administered saline (10 ml/kg, i.p.) or microcystin (50 μg/kg, i.p.).

Livers were removed 8-PCR. Values are expressed as mean ± S.E.M. (n = 8). *Significantly different from the basal level of the same genotype (p ≤ 0.05); #Significantly different from Nrf2-null mice treated with microcystin (p ≤ 0.05). Representative Western-blot analysis of Nqo1 and Gclc proteins inserted into mRNA expression graphs. Nqo1 and Gclc were higher in Keap1-HKO mice at both basal levels and after microcystin.