Table 5. Oxidative biomarkers and antioxidant enzymes status in the liver of barrows.
Items | Control (n = 6) | Daidzein (n = 5) | D+L (n = 6) | P |
Total carbonyl (mg/g wt liver) | 7.07±0.78a | 4.69±1.10b | 5.09±1.04b | 0.002 |
Hydrogen Peroxide(H2O2) (mmol/g protein) | 5.77±0.65 | 5.50±0.81 | 5.40±0.88 | 0.699 |
Inhibiting Hydroxyl Radical (U/mg protein) | 25.15±6.34 | 26.19±3.47 | 30.42±6.52 | 0.280 |
NO (μmol/g protein) | 0.41±0.04 | 0.32±0.05 | 0.36±0.07 | 0.079 |
Protein Carbonyl (nmol/mg protein) | 5.16±0.73 | 4.24±0.80 | 4.40±0.44 | 0.076 |
8-OH-dG (ng/g wt liver) | 337.00±47.43a | 258.53±75.22b | 244.54±26.67b | 0.017 |
CAT (U/g protein) | 78.34±10.54 | 78.34±9.68 | 84.23±8.93 | 0.509 |
Total-SOD (U/mg protein) | 239.08±12.06b | 251.43±15.11b | 299.38±35.46a | 0.002 |
CuZn-SOD (U/mg protein) | 156.04±13.49ab | 134.32±5.90b | 169.73±29.03a | 0.030 |
Mn-SOD (U/mg protein) | 83.04±5.36b | 117.11±15.13a | 129.65±14.96a | <0.0001 |
MDA (nmol/mg protein) | 2.62±0.51 | 2.27±0.59 | 2.11±0.30 | 0.174 |
GSH-px (activity unit) | 20.08±1.56 | 21.70±2.13 | 20.49±20.70 | 0.417 |
GR (U/g protein) | 7.34±2.07 | 5.81±1.33 | 6.17±1.50 | 0.310 |
GSH (μmol/g wt liver) | 0.84±.09 | 0.81±0.10 | 0.77±0.07 | 0.383 |
GSSG (μmol/g wt liver) | 0.17±0.03 | 0.17±0.02 | 0.19±0.02 | 0.300 |
GSH/GSSG | 5.1±0.98 | 4.74±0.92 | 4.05±0.34 | 0.090 |
Note: values with different small letter superscripts in the same row mean that there was a significant difference (P<0.05), and the same small letter superscripts mean that there was no difference.