Figure 1. Neuroprotective effect of NX210 against H₂O₂ using a B104 cell line.

Neuroprotective effect of NX210 at 100 and 250 μg/ml was assessed by the resistance of a B104 neuroblastoma cell line after 18 hours of exposure to H₂O₂. (A) Morphological observation using phase-contrast microscopy shows normal features of B104 cells in the control group. Exposure to H₂O₂ alone decreases B104 cell viability, normal B104 cells are partly replaced by numerous cellular debris. Treatment with NX210 250 μg/ml significantly prevents H₂O₂-induced cell death. (B) Viability of B104 cells treated with NX210 was assessed using the WST-1 assay. H₂O₂-induced cell death was significantly prevented in NX210-treated cultures at the concentration of 250 μg/ml. Each experiment was performed in triplicate and repeated 4 times. Data represent the percentage of live cells relative to controls (no treatment) and are presented as mean values +/- SEM. * significantly different from H₂O₂-treated cells, p<0.001. Scale bar  =  100 μm.