Figure 2. In vivo effects of NX210 on neuritic regrowth in a rat model of SCI.

The injury was performed by aspirating the dorsal funiculi and the dorsal horns of the spinal gray matter. A collagen tube filled with the vehicle (A) or NX210 (B and C) was placed into the site of injury. Neuritic regrowth was detected using neurofilament immunostaining. (A) In the vehicle group (n = 5), the collagen tube remains empty without neuritic regrowth (*). (B and C): in the NX210 group (n = 5), neuritic regrowth (arrow) is clearly observed inside the collagen tube, mostly from the caudal end of the lesion. Neofibers display a longitudinal arrangement. (D): At the surface of the growing processes, NF was co-localized with laminin, a marker of the basal lamina. r: rostral; c: caudal; Scale bar: 250 μm.