Figure 1. Total IgG and anti-dsDNA IgG ASCs accumulate with age and are highly enriched in the kidney in lupus prone mice.

39 week NZB/W F1 female mice with high titer of dsDNA antibody (High) (n=5, >5-fold higher compared with that in 24 week C57BL/6 mice) were compared to that with low titer of dsDNA antibody (Low) (n=4, <3-fold higher compared with that in 24 week C57BL/6 mice). To determine the kinetics of ASC development, 8 week (n=5) and 19 week (n=5) NZB/W F1 female mice were used. Total cells from spleen, bone marrow (BM) and kidney were harvested and ELISpot assay was performed as described in “materials and methods”. Horizontal bar represent mean (long) and SEM (short). A. total IgG ASCs in spleen, BM, and kidney. B. anti-dsDNA IgG ASCs from spleen, BM and kidney. Anti-dsDNA IgG ASCs were undetectable in 8 week NZB/W F1 female mice. C. the ratio of anti-dsDNA IgG ASCs/total IgG ASCs in spleen, BM, and kidney, respectively. Left scale for spleen and BM, right scale for kidney. Significant p values (p<0.05) (unpaired t-test as follows:

*significantly different from the other groups

^significantly different from high

#significantly different from high and 8 wk

**significantly different from corresponding group in spleen and BM