Skip to main content
. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Immunol. 2014 Feb 26;192(7):3011–3020. doi: 10.4049/jimmunol.1302003

Figure 3. Changes in plasma cell survival and chemotactic factors in spleen and kidney.

Figure 3

Total RNA was isolated from spleens (A) and kidney (B) from Balb/C control mice and NZB/W mice at the indicated age. cDNA was prepared with superscript II and random primers and concentration adjusted to 10 ng/ul. 50 ng of cDNA were used for quantitative PCR reactions using specific probes for BAFF, APRIL, CXCL12 and IL6. Levels of expression for each gene were first normalized to GAPDH and then compared to the levels of expression in Balb/C mice, according to the ΔΔCT method. Representative data from two experiments with similar results is shown. N=3–5 mice per group. Bars show mean±SEM. p values are calculated by Mann-Whitney test. C. Increase in protein expression of PC survival factors in the kidney in NZB/W mice. 4 μm frozen kidney sections were probed with antibodies against BAFF (red), APRIL (red), CXCR12 (green) and IL6 (red). Representative pictures from kidney of each group (n=5) are shown. Quantitation of BAFF, APRIL, CXCL12 and IL6 expression was performed by morphometric analysis. Bars show mean±SEM. p values are calculated by unpaired t- test.