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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Genesis. 2014 Jan 17;52(3):279–286. doi: 10.1002/dvg.22737

Figure 2.

Figure 2

Nanos regulation in the sea urchin at the mesenchyme blastula stage. (A) Schematic of the Sp-nanos2 transcript: it contains a cap, Sp-nanos2 5’UTR, Sp-nanos2 ORF and Sp-nanos2 3’UTR. The regulatory elements, GNARLE and NRRE, located in Sp-nanos2 3’UTR, are presented with a red and a blue line respectively. To test nanos regulation, Sp-nanos2 ORF was exchanged by the GFP ORF, and the 3’UTR was modified. Constructs were injected in Sp fertilized eggs. (B) Schematic of the GFP protein expression and the retention of the RNA obtained in mesenchyme blastula after injection of the construct presented in (A). Four different 3’UTRs were tested: Sp-nanos2 3’UTR full length, GNARLE, ΔGNARLE, and Xenopus β-globin 3’UTR.