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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Immunol. 2014 Feb 24;192(7):3057–3067. doi: 10.4049/jimmunol.1302063

Figure 5. Chromatin characteristics upstream of TNF.

Figure 5

Chromatin marks were compared in K562 and THP1 cell lines. ChIP assays showed that A) H3K27me3, B) EZH2, and C) Suz12 were increased in K562 cells compared to THP1 cells. N=5. D) LRRFIP1 was also found to bind to the same region of TNF promoter in K562 cells. N=5. Error bars in all panels denote SE. * indicates the P value of K562 compared with THP1 cells for the same antibody. One asterisk (p<0.05); two asterisks (p<0.01), three asterisks (p<0.001). E) Co-immunoprecipitation analysis of the interaction of LRRFIP1 with SUZ12 and EZH2 in K562 cells. The lysates were precipitated with anti-IgG, EZH2 or SUZ12 and analyzed by western blot with LRRFIP1 antibodies. This is one representative western blot from three experiments.