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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Hum Pathol. 2013 Oct 31;45(4):880–883. doi: 10.1016/j.humpath.2013.10.016

Figure 1. Schematic of BEAMing.

Figure 1

DNA is diluted and partitioned into emulsions with magnetic beads such that single or no DNA template molecules are present within each emulsion. Emulsions also contain primers, thermostabile DNA polymerase, dNTPs and other PCR reagents. Emulsions are then amplified massively in parallel and then the emulsions are burst open followed by hybridization to mutant and wild type specific fluorescent probes. Amplified DNA on beads are then quantified using flow cytometry to determine the number of mutant and wild type DNA molecules present.