Figure 2. Pathology and sequencing analysis of tumor specimens.
FFPE samples were prepared for routine hematoxylin and eosin staining and photographed under 20× magnification. In addition, genomic DNA (gDNA) was extracted from serial sections obtained from FFPE tumor blocks and then used for Sanger sequencing. Shown are representative sections from the patient’s A: breast cancer, and B: lung cancer. C: PIK3CA Exon 9 Sequencing results are shown for breast cancer gDNA harboring the mutation (left panel) and lung cancer gDNA that is wild type for PIK3CA (right panel).