(A) Airway phagocyte profiles (3 mice/group), and (B) intracellular ROS levels (3 mice/group) at 24 h after infection with 107 CFU of MRSA BAA-1695 alone (MRSA) or on day 7 after PR8 infection (Flu + MRSA). In (A&B), P<0.001, ANOVA; *P< 0.05, ***P< 0.001, Tukey’s multiple comparisons test. Control mice were inoculated with only PBS (PBS) or infected with 50 PFU PR8 and inoculated with PBS (Flu) 7 days after PR8 infection. The data are representative of two independent experiments.