Numbers of bacteria remaining in the lungs 24 h after MRSA BAA-1695 infection of C57BL/6 (A) WT, NOS2−/−, gp91phox−/−, and p47phox−/− mice, (B) α-PMN antibody-treated WT and gp91phox−/− mice, and (C) Mafia mice. In (B), mice were treated with RB6-8C5 mAb for neutrophil depletion. In (C), Mafia mice were depleted of macrophages using AP20187 dissolved in ethanol. Control WT mice were also treated with AP20187. (D) Survival of α-PMN antibody-treated WT mice after i.n. infection with 108 CFU of MRSA. Control WT mice were treated with Rat IgG. (E) Survival of α-PMN antibody-treated WT and gp91phox−/− mice after i.n. infection with 107 CFU of MRSA. In (A, B&C), P<0.001, ANOVA; ***P< 0.001, Tukey’s multiple comparisons test.