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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Microbes Infect. 2013 Nov 26;16(3):225–236. doi: 10.1016/j.micinf.2013.11.007

Fig. 3. A profilin mutant defective in binding actin has lost its ability to suppress the toxicity of Ceg14.

Fig. 3

A. The yeast strain inducibly expressing Ceg14 was transformed with plasmids that direct the production of Flag-tagged human profilin, yeast profilin or its mutants defective in binding actin, phosphoinositol or poly-l-proline. The strains were then grown in glucose medium before spotting dilutions onto glucose and galactose plates. Images were acquired after 3-day incubation at 30°C. B. The expression of Ceg14, profilin and its mutants were probed in yeast cells induced by galactose for 6 hrs. Ceg14 was detected with a specific antibody and the two profilin proteins and mutants were detected by using the Flag-specific antibody. The 3-phosphoglycerate kinase (PGK) was also probed as a loading control (lower panel). Protein size makers (in kDa) are on the left lane of the blot.