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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Microbes Infect. 2013 Nov 26;16(3):225–236. doi: 10.1016/j.micinf.2013.11.007

Fig. 7. Kinetics of the inhibition of actin polymerization by Ceg14.

Fig. 7

Monomeric actin containing 10% pyrene-labeled actin was mixed with indicated testing proteins the general actin buffer. The intensity of fluorescence signals was monitored by a fluorescence spectrophotometer after initiating actin polymerization. A. Dose-dependent inhibition of actin polymerization by Ceg14. B. Non-toxic mutants of Ceg14 have lost the ability to inhibit actin polymerization. C. PFN did not interfere with the inhibitory effect of Ceg14. Note that addition of PFN did not alleviate the activity of Ceg14.