Fig. 1.

Changes in mRNA expression of StAR (A) and Cyp19a1 (B) in granulosa cells undergoing luteinization during ovulation. Granulosa cells were obtained from rats treated with equine chorionic gonadotropin before (0 hours) and 4, 8, and 12 hours after human chorionic gonadotropin injection. mRNA levels were measured by real-time reverse transcription polymerase chain reaction. Data were expressed as a ratio of StAR/Cyp19a1 mRNA to GAPDH (internal control). Each bar represents the mean ± SEM of five animals. The data were reported in the reference no. 15.