Fig. 2.

DNA methylation statuses of the promoters of StAR (A) and Cyp19a1 (B) in granulosa cells undergoing luteinization during ovulation. The diagram shows the distribution of CpG sites and location of the response elements for transcriptional factors, in which the tick marks indicate the positions of CpG sites. The position of the transcription start site is designated as +1. DNA methylation statuses of the CpG sites were analyzed by sodium bisulfite genomic sequencing in the StAR promoter region (-13 to -1772 bp) and Cyp19a1 promoter region (-68 to -1079 bp). Granulosa cells were obtained from rats treated with equine chorionic gonadotropin before (0 hours) and 4 hours, and 12 hours after human chorionic gonadotropin (hCG) injection. The data before hCG (0 hours) were only shown because the methylation statuses did not change at any time observed after hCG injection. Open and filled circles indicate unmethylated and methylated CpG status, respectively. The data were reported in the reference no. 15. SF-1/Ad4BP, steroidogenic factor 1/adrenal 4 binding protein; GATA-4, GATA binding protein 4; C/EBP β, CCAAT/enhancer binding protein β; CLS, cAMP-responsive element-like sequence.