Figure 10.

Signaling changes in Raptor and Rictor cKO CNS. Immunohistochemical localization of signaling proteins in oligodendrocytes in P14 (A) and P29 (B) spinal cord sections of floxed control (left), Raptor cKO (middle), and Rictor cKO (right) mice. Sections were analyzed for expression of pS6RP, pERK, or pRaptor in CC1-, Olig2-, or Sox10-positive cells (A, B). Representative Western blots of whole spinal cord or corpus callosum-enriched lysates of P14 and P29 Raptor (C, D) or Rictor (E, F) cKO mice. For significance of signaling proteins that were unchanged from controls or that were significantly decreased, see Tables 3, 4. Schematic of a proposed model for signaling mechanisms regulating myelination within the oligodendrocyte lineage (G). Scale bars: A, B, 50 μm.