Table 4.
Western blot quantification of Rictor fl/fl and cKO animal signaling proteins
| fl/fl % | Rictor spinal cord |
Rictor corpus callosum |
|||
|---|---|---|---|---|---|
| P14 (% control with p value) | P29 (% control with p value) | P14 (% control with p value) | P29 (% control with p value) | ||
| pAkt308/Akt | 100 | 81.47 ± 10.51 | 107.85 ± 20.08 | 101.68 ± 19.21 | 92.42 ± 6.76 |
| pAkt473/Akt | 100 | 83.84 ± 11.24 | 88.80 ± 13.02 | 123.79 ± 9.57* | 67.40 ± 14.45* |
| pERK/ERK | 100 | 175.00 ± 82.73 | 101.68 ± 19.21 | 88.61 ± 27.33 | 150.39 ± 88.90 |
| pS6RP/β-actin | 100 | 25.46 ± 0.18**** | 163.77 ± 22.98* | 24.56 ± 2.33**** | 68.54 ± 4.48** |
| pmTOR2448/mTOR | 100 | 63.43 ± 1.3**** | 83.56 ± 10.59 | 119.65 ± 12.71 | 60.95 ± 5.98** |
| pmTOR2481/mTOR | 100 | 42.19 ± 15.37** | 30.6 ± 6.65**** | 97.01 ± 11.93 | 111.01 ± 16.08 |
| Raptor/β-actin | 100 | 56.57 ± 8.26** | 108.30 ± 8.09 | 74.41 ± 14.33 | 97.78 ± 4.48 |
| Rictor/β-actin | 100 | 0.90 ± 0.43**** | 22.5 ± 5.71**** | 37.32 ± 2.77**** | 16.42 ± 4.31**** |
Lysates prepared from P14 and P29 whole spinal cord lysates or white matter-enriched regions of the corpus callosum (see Results). Values ±SEM, n = 3,
*p ≤ 0.05,
**p ≤ 0.005,
***p ≤ 0.001,
****p ≤ 0.0001.