Table 1. Details of Amplified Region and Primer Sets Used for PCR Analysis of Recombinant Bacmid.

Primer Pairs	Sequence (5' to 3')	Fragment a Size (bp)	The Amplified Region
pUC/M13 F M2e/ctxB R	CCCAGTCACGACGTTGTAAAACG CGGAATTCTTAATTTGCCATACTAATTG	2200	Tn7 R + Polyhydrin promoter + M2e/ctxB fusion gene
M2e/ctxB F pUC/M13 R	CGGGATCCACCATGTCCCTGCTGACCGAGG AGCGGATAACAATTTCACACAGG	1200	M2e/ctxB fusion gene + Tn7 L
pUC/M13 F pUC/M13 R	CCCAGTCACGACGTTGTAAAACG AGCGGATAACAATTTCACACAGG	2900	Tn7 R + Polyhydrin promoter + M2e/ctxB fusion gene + Tn7 L
M2e/ctxB F M2e/ctxB R	CGGGATCCACCATGTCCCTGCTGACCGAGG CGGAATTCTTAATTTGCCATACTAATTG	460	M2e/ctxB fusion gene

^a All the Fragment Sizes are Expressed Approximately and Has Been Calculated According to the Sequence date of the Bacmid DNA