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. 2014 Apr;349(1):56–65. doi: 10.1124/jpet.113.210708

Fig. 9.

Fig. 9.

Selective PDE4 inhibition with rolipram significantly reduces 20 Hz EFS-induced contractions of DSM-isolated strips in a concentration-dependent manner. (A) A representative recording of a DSM strip showing the concentration-dependent inhibitory effects of rolipram (0.1 nM-10 µM) on EFS-induced contractions. Post-treatment of the DSM strip with paxilline (1 μM) recovered the EFS-induced contractions. (B–D) Cumulative concentration-response curves for the inhibitory effects of rolipram on DSM contraction amplitude (B), muscle force integral (C), and muscle tone (D) (n = 10, N = 6; *P < 0.05, versus control). (E) Summary data illustrating that addition of 1 μM paxilline following application of 10 µM rolipram significantly recovered the EFS-induced contraction amplitude and muscle force in DSM-isolated strips (n = 8, N = 5; #P < 0.05 paxilline versus 10 μM rolipram; P > 0.05; NS, nonsignificant). Amp, amplitude; pax, paxilline; Rol, rolipram.