TABLE 1.
Coprecipitation of Gα12 class-distinctive mutants by downstream effectors
For each class-distinctive mutant of myc-Gα12QL (left column), precipitation by each GST-fused effector protein (top row) and starting material (i.e., load) were quantified as described in Materials and Methods to determine a precipitate:load ratio. Categories are as follows: ++++, ratio >70% of positive control myc-Gα12QL; +++, 40–70% of control; ++, 20–40% of control; +, 0–20% of control; −, no detectable coprecipitation. Each sample is the result of two trials, except for samples scoring ++, +, or –, which were analyzed in at least three trials.
| p115 | LARG | Hsp90 | |
|---|---|---|---|
| A30E | ++++ | ++++ | ++/+++ |
| A43K | +++ | +++ | +++ |
| E82G | ++++ | ++++ | ++++ |
| A87E | ++++ | ++++ | ++++ |
| R110M | ++++ | ++++ | ++ |
| A164C | ++/+++ | +++ | ++++ |
| L201R | ++++ | +++ | −/+ |
| K204V | +++ | +++ | ++/+++ |
| K207T | ++++ | ++++ | ++ |
| QE/QK | +++ | ++++ | −/+ |
| F237I | ++/++++a | ++++ | + |
| I243V | ++/+++ | +++ | ++++ |
| S251A | ++ | ++++ | +++ |
| V325A | +++ | +++/++++ | +++ |
| T350C | ++ | ++ | ++ |
a
Variation spanning more than two categories.