Fig. 3.

Effect of C. rodentium infection on hepatic mRNA expression of bile acid–associated genes. Seven days following oral infection with C. rodentium, female mice were sacrificed, livers harvested, and mRNA isolated from (A) HeOuJ and HeJ (TLR4-null) mice (n = 6) and (B) C57BL/6, IL-6-null, and IFNγ-null mice (n = 8). mRNA was quantified by real-time qRT-PCR and resulting values are expressed as relative levels of mRNA expression after normalization to GAPDH, with uninfected wild-type (HeOuJ or C57BL/6) set to 1. Values represent mean ± S.E.M. Significant differences are in comparison with uninfected control groups. *P < 0.05 by t test.