Fig. 2.

ERα transactivates SULT2A1 promoter. (A) HepG2-ER cells were treated with vehicle (ethanol) or E2 (100 nM) in the presence or absence of cycloheximide (CHX; 10 μg/ml) for 16 hours. SULT2A1 mRNA expression was determined by qRT-PCR. **P < 0.01; ***P < 0.001. (B) HepG2 cells were cotransfected with pGL3-SULT2A1 (–826/+140), ERα expression plasmid (or pcDNA3 empty vector), and pCMV-Renilla. The transfected cells were treated with vehicle (ethanol) or E2 (1–100 nM) for 24 hours, and luciferase assay was performed. ***P < 0.001 versus vehicle treatment.