Fig. 4.

AP-1 proteins bind to S2. Nuclear proteins from HepG2-ER cells treated with vehicle or E2 for 45 minutes were prepared. The proteins were incubated with ³²P-labeled DNA probes harboring S2 or consensus AP-1 binding sequence (shown at the bottom) in the presence or absence of various antibodies or unlabeled DNA probes as competitors (in 100-fold excess). The mixture was resolved on nondenaturing gel. The lower arrow indicates the location of shifted bands by apparent AP-1 binding to DNA. The upper arrow indicates the super-shift complex.