. Author manuscript; available in PMC: 2015 Jan 1.

Published in final edited form as: Biophys Chem. 2013 Nov 13;185:58–69. doi: 10.1016/j.bpc.2013.11.002

Table 2.

ClpA Polypeptide Translocation Parameters as a Function of [ATPγS]

[ATPγS] (µM)	m (aa step⁻¹)	k_T (s⁻¹)	mk_T (aa s⁻¹)	k_NP (s⁻¹)	k_C (s⁻¹)
75	16 ± 1	1.3 ± 0.1	20.8 ± 0.4	0.039 ± 0.002	0.15 ± 0.01
126	17 ± 1	1.2 ± 0.1	20.4 ± 0.1	0.034 ± 0.001	0.141± 0.004
250	17 ± 2	1.1 ± 0.1	18.6 ± 0.2	0.031 ± 0.001	0.125 ± 0.003
355	17 ± 2	1.1 ± 0.2	17.6 ± 0.4	0.029 ± 0.001	0.118 ± 0.003
600	16 ± 2	1.0 ± 0.1	15.7 ± 0.6	0.028 ± 0.001	0.102 ± 0.004
[ATPγS] (mM)
1.0	16 ± 1	0.8 ± 0.1	13.1 ± 0.3	0.03 ± 0.01	0.089 ± 0.003
1.8	20 ± 3	0.5 ± 0.1	10.7 ± 0.3	0.0163 ± 0.0004	0.052 ± 0.001
2.5	24 ± 6	0.4 ± 0.1	9.1 ± 0.3	0.015 ± 0.001	0.043 ± 0.004

k_T is the translocation rate constant, k_C is an additional kinetic step defined by Scheme 1, m is the kinetic step size, k_NP is a slow conformational change defined by Scheme 1, and mkT is the macroscopic rate of translocation