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. 2014 Mar 26;4:4469. doi: 10.1038/srep04469

Figure 7. Mitochondrial p53 activates mitochondrial apoptosis pathway in PFT treated HUVEC cells.

Figure 7

HUVEC cells were pretreated with or without pifithrin-α for 1.5 h followed by exposure to 43°C for 2 h, and the cells further incubated for 6 h. (a–b) Western blot(cropped) analysis of the effect of PFT on heat stress-induced P53 mitochondrial translocation and release of cytochrome c in HUVEC cells. COX IV, mitochondrial loading control. (c) The effect of PFT on enzymatic activity of caspase-9 was measured in cell lysate using fluorogenic substrate Ac-LEHD-AFC and was expressed relative to the control at 37°C (100%). (d) Flow cytometry and JC-1 measure the effet of PFT on ΔΨm. Data are presented as mean ± SD for three independent experiments, *P < 0.05 compared with HS group.