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. 2014 Mar 11;4(3):e371. doi: 10.1038/tp.2014.13

Figure 4.

Figure 4

Loss of parvalbumin (PV)-positive γ-aminobutyric acidergic (GABAergic) interneurons in the medial prefrontal cortex (mPFC) of maternally stressed P21 GAD67+/GFP offspring. (a) Region of analysis in the mPFC of a coronal section at P21 (left, dotted square). BrdU was injected at E15.0 immediately before maternal stress (MS). Middle panels are BrdU and green fluorescent protein (GFP) double-positive cells at higher magnification in the region indicated in the left panel (BrdU/GFP). Right panels (PV/GFP) show PV and GFP double-positive cells in the mPFC. Bars=200 μm. Note the reduction in double-positive cells in stressed offspring. (b) Quantitative analysis of BrdU and GFP double-positive cells in the mPFC at P21. The density of BrdU(+)/GFP (+) cells from the stressed group was significantly lower than that of the control (**P<0.01, t-test; control, n=6 sections from three brains; stress, n=7 sections from four brains). (c) Quantitative analysis of BrdU-negative and GFP-positive cells in the mPFC. There was no significant difference between control and stressed pups (P=0.68, t-test; control, n=6 sections from three brains; stress, n=7 sections from four brains). (d) Quantitative analysis of PV and GFP double-positive cells in the mPFC at P21. The density of PV (+)/GFP (+) cells in the stress group was significantly lower than that of the control (***P<0.001, t-test; control, n=5 sections from three brains; stress, n=5 sections from four brains). Error bars represent the s.e.m.