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. 2014 Feb 15;4(5):487–497. doi: 10.7150/thno.8263

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Uptake of CP-MSNP/Alexa555 siRNA by MDA-MB-231 cells. (A) Time-dependent analysis of CP-MSNP/Alexa555 siRNA (red) inside MDA-MB-231 cells. Final siRNA concentration: 50 nM. (B) Quantitative flow cytometry analysis of Alexa555-positive cells after incubation with CP-MSNP/Alexa555 siRNA at different time points. Untreated cells were used as a negative control. Final siRNA concentration: 50 nM. (C-D) MTS assay on cell viability following incubation with CP-MSNP (C) or CP-MSNP/siRNA (D) for 72 h. Results are presented as the mean of three measurements ± standard deviation.