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. Author manuscript; available in PMC: 2014 Mar 26.
Published in final edited form as: Circ Res. 2012 Jul 9;111(5):553–563. doi: 10.1161/CIRCRESAHA.112.274548

Figure 5.

Figure 5

SNO disrupts the association between β-catenin and p120. Protein extracts from ECV-GFPeNOS-G2A and ECV-GFPeNOS-CAAX cells, treated with 5×10-7 mol/L PAF for different times, were immunoprecipitated (IPP) with anti β-catenin antibodies and probed by Western blot with anti-p120 antibodies. A) Experiments in ECV-GFPeNOS-G2A cells. A representative Western blot assessing the association between β-catenin and p120 in ECV-GFPeNOS-G2A cells treated with PAF for different times is shown on the upper section, with corresponding statistical analysis illustrated below. Time indicates the duration of PAF application. * P < 0.05 as compared with control; n = 3. B) Experiments in ECV-GFPeNOS-CAAX cells. The upper section shows a representative Western blot assessing whether or not PAF stimulates the disruption of the association between β-catenin and p120 in ECV-GFPeNOS-CAAX cells. The statistical evaluation is illustrated in the lower section. No statistical significance was observed.