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. Author manuscript; available in PMC: 2014 Mar 26.
Published in final edited form as: Circ Res. 2012 Jul 9;111(5):553–563. doi: 10.1161/CIRCRESAHA.112.274548

Figure 8.

Figure 8

Mapping of S-nitrosation sites in p120. Protein sequence from human p120. Purified p120 was S-nitrosated with GSNO for 30 minutes, subjected to biotin switch assay followed by in-solution trypsin digest. The region that potentially interacts with VE-cadherin is shown in gray background. Cysteine 579 (white letter, red background) was 100% S-nitrosated according to mass spectrometry. Other cysteines (red background) were not S-nitrosated in 100% of the assays. Unlabeled cysteines were not S-nitrosated. (See Supplemental Data for experimental details).