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. Author manuscript; available in PMC: 2014 Aug 25.
Published in final edited form as: Nat Commun. 2014 Feb 25;5:3338. doi: 10.1038/ncomms4338

Figure 6. Induction of NSCs from spinal astrocytes by SOX2 in culture.

Figure 6

(a) Experimental scheme. (b) Immunocytochemistry confirming the astrocyte identity of cultured tdT+ cells. (c) Representative micrographs of neruospheres from spinal astrocytes infected with the hGFAP-GFP-T2A-SOX2 lentivirus (dpi, days post infection). (d) Frequency of neurosphere-forming cells from spinal astrocytes infected with the indicated lentivirus. Secondary spheres were quantified at 7 days after plating (mean+s.d.; n=5; *p < 0.01 by Student’s t-test). (e) Nestin (NES) expression in SOX2-induced neurospheres. (f) Multiple lineage differentiation of SOX2-induced secondary neurospheres. Immunocytochemistry was performed 6 days after plating dissociated single cells. Scales: 50 µm (b), 200µm (c), 60 µm (e), and 40 µm (f).