Figure 2. Peritoneal B cells derived from Lin^-CD45R^-CD19^- fetal liver cells phenotype as B-1a cells.

Peritoneal IgM^a+CD45R^loCD5⁺ cells derived from Lin^-CD45R^-CD19^- fetal liver cells were analyzed for surface markers typically found on peritoneal B-1a cells. These cells were compared to peritoneal B-1a and splenic B-2 cells from 3-month old BALB/c-ByJ mice in addition to peritoneal IgM^a+CD45R^loCD5⁺ cells derived from Lin^-CD45R^lo/-CD19⁺ fetal liver cells. (A) MFI values of IgD and CD19 are shown. (B) Percent of peritoneal IgM^a+CD45R^loCD5⁺ cells or splenic IgM^a+CD45R^highCD5^- cells expressing CD43, Mac-1 (CD11b), or CD23 are shown. (C) Percent of peritoneal IgM^a+CD45R^loCD5⁺ cells or splenic IgM^a+CD45R^highCD5^- cells expressing CD86, PD-L2, or PtC liposome binding are shown. The data shown are an average of six independent experiments with 3-5 mice per group in each experiment.