Figure 4. Immunoglobulin heavy chain sequence analysis of Lin^-CD45R^-CD19^- fetal liver and Lin^-CD45R^lo/-CD19⁺ bone marrow derived B-1a cells show abundant N-addition.

(A) N-region addition analysis at the D-J and V-D junctions in B-1a cells derived from Lin^-CD45R^-CD19^- and Lin^-CD45R^lo/-CD19⁺ fetal liver cells, Lin^-CD45R^lo/-CD19⁺ bone marrow cells, and total Lin- bone marrow is shown. (B) V, D, and J gene segment usage in B-1a cells derived from Lin^-CD45R^-CD19^- or Lin^-CD45R^lo/-CD19⁺ fetal liver cells is displayed. (C) Average number of N-additions at the V-D, D-J, or sum of the two junctions is shown along with CDR3 length. Results are based on 3-5 independent experiments with sequences combined from each independent experiment.