Figure 1.
A, Aurora A expression in human neuroblastoma cell lines determined by quantitative RT-PCR (i) and Western blot analysis (ii). Western blots were probed with anti-Aurora A antibody and reprobed with anti-β-actin antibody to show equal protein loading. Breast tumor cell line MCF-7 and normal breast epithelial cell line MCF-10A were used as positive and negative controls for Aurora A expression, respectively. B, analysis of Aurora A expression in neuroblastoma and ganglioneuroma tumor tissue samples by Western blot. Representative neuroblastoma tumor samples are shown, grouped by INSS stage.