Table 2.
Biological characteristics and sensitivity to MLN8054 in human neuroblastoma cell lines
| Cell line | Origin | MYCN | p53 status | Cell type | IC50 of MLN8054 (µmol/L) |
|---|---|---|---|---|---|
| SK-N-SH | NB tumor | SC | WT | N + S | 0.46 ± 0.01 (2) |
| SH-SY5Y-Luc | NB tumor | SC | WT | N | 0.24 ± 0.02 (4) |
| IMR-32 | NB tumor | A | WT | N | 0.30 ± 0.08 (2) |
| LAN-1 | NB tumor | A | Null | N | 0.67 ± 0.04 (4) |
| JF | NB tumor | A | N/D | N | 0.26 ± 0.06 (3) |
| SH-EP | NB tumor | SC | WT | S | 0.92 (1) |
| NB19 | NB tumor | A | WT | S | 0.50 (1) |
| SMS-KCN | NB tumor | A | N/D | S | 2.77 ± 0.18 (2) |
| SK-N-AS | NB tumor | SC | mt | S | 4.11 ± 1.03 (2) |
| MCF-10A | Breast normal epithelial | WT | 12.92 (1) | ||
| MCF-7 | Breast tumor | WT | 0.44 (1) |
NOTE: Numbers represent average ± SD IC50 derived from a Cell Counting Kit-8 proliferation assay. Values in parentheses represent the number of experiments completed for each cell line.
Abbreviations: NB, neuroblastoma; SC, single copy; A, amplified; WT, wild-type; mt, mutated; N/D, not described; N, neuroblastic; S, substrate adherent.